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M9480108.TXT
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1994-08-09
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Document 0108
DOCN M9480108
TI Quantifying HIV-1 proviral DNA using the polymerase chain reaction on
cerebrospinal fluid and blood of seropositive individuals with and
without neurologic abnormalities.
DT 9410
AU Schmid P; Conrad A; Syndulko K; Singer EJ; Handley D; Li X; Tao G;
Fahy-Chandon B; Tourtellotte WW; Neurology Service, DVA Medical Center
West Los Angeles, Wadsworth; Division, California.
SO J Acquir Immune Defic Syndr. 1994 Aug;7(8):777-88. Unique Identifier :
AIDSLINE MED/94293159
AB To quantify the number of human immunodeficiency virus type 1 (HIV-1)
proviral copies per 1,000 CD4+ cells in cerebrospinal fluid (CSF) and
blood in relationship to stage of infection and HIV-1 neurologic disease
(HND), 87 HIV-1 seropositive men without CNS opportunistic infections,
tumors, or neurosyphilis, 9 high-risk, and 14 not-at-risk seronegative
controls underwent a structured interview, and physical and neurologic
examination followed by blood and CSF collection. A custom-designed,
fully automated polymerase chain reaction (PCR) system performed
amplification with use of two HIV-1 gag primer pairs, Southern blotting,
and hybridization with product-specific probes. Image analysis was used
to quantify band intensities relative to a dilution series. Eighty-one
of 87 (93%) seropositive patients, 1 of 9 high-risk patients, (11%) and
none of 14 seronegative controls had PCR-detectable HIV-1 in their
blood. Fifty-seven of 63 (90%) seropositive patients, 2 of 5 (40%)
high-risk seronegative patients, and none of 14 controls had HIV-1 in
their CSF. The proviral load in seropositive patients, all stages, was
significantly greater in CSF than blood [median 25 vs. 0.6 copies/1,000
CD4+ cells (p = 0.0001)]. The median proviral load in blood was 0.09
copies/1,000 CD4+ cells in seropositive, asymptomatic subjects, 10.7 in
patients with AIDS, and 1.4 in patients with AIDS-related complex (p =
0.0281). CSF proviral load was greater in seropositive patients with HND
than those without HND, median 43.5 vs. 17.6 copies/1,000 CD4+ cells (p
= 0.0614). Proviral load was greater in the blood and CSF of subjects
with more advanced systemic disease and HND. There was a substantial
penetration of HIV-1 into the CNS/CSF in both systemically and
neurologically asymptomatic HIV-1 disease.
DE Adult AIDS Dementia Complex/BLOOD/CEREBROSPINAL FLUID/DIAGNOSIS Base
Sequence Blotting, Southern Cerebrospinal Fluid/CYTOLOGY/MICROBIOLOGY
DNA Primers/CHEMISTRY DNA, Viral/*BLOOD/*CEREBROSPINAL FLUID Human
HIV Infections/BLOOD/CEREBROSPINAL FLUID/*DIAGNOSIS
HIV-1/*GENETICS/ISOLATION & PURIF Male Molecular Sequence Data
Polymerase Chain Reaction Prognosis Proviruses/*GENETICS/ISOLATION &
PURIF Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. T4
Lymphocytes/MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).